Certified Tissue Bank Specialist (CTBS) Practice Test 2025 – 400 Free Practice Questions to Pass the Exam

Most tissues can be stored in cryopreservation indefinitely under optimal conditions. This method of preservation involves cooling biological samples to extremely low temperatures, typically using liquid nitrogen. At these temperatures, biochemical reactions slow down significantly, and cellular metabolism is essentially halted. As a result, the cells remain viable for extended periods without undergoing the degradation that would typically occur at higher temperatures.

Optimal conditions include maintaining a consistent low temperature, using suitable cryoprotectants to prevent ice crystal formation, and ensuring that the storage environment is carefully monitored to avoid temperature fluctuations. When these conditions are met, tissues can remain preserved effectively for indefinite periods, making cryopreservation a crucial technique for long-term storage in tissue banking and regenerative medicine.

In contrast, the other time frames suggested (a few weeks, up to one year, or a maximum of five years) do not capture the potential longevity of tissue viability that cryopreservation can offer under ideal conditions.